Dual-wavelength photothermal optical coherence tomography for imaging microvasculature blood oxygen saturation


A swept-source dual-wavelength photothermal (DWP) optical coherence tomography (OCT) system is demonstrated for quantitative imaging of microvasculature oxygen saturation. DWP-OCT is capable of recording three-dimensional images of tissue and depth-resolved phase variation in response to photothermal excitation. A 1,064-nm OCT probe and 770-nm and 800-nm photothermal excitation beams are combined in a single-mode optical fiber to measure microvasculature hemoglobin oxygen saturation ($SO_2$) levels in phantom blood vessels with a range of blood flow speeds (0 to 17 mm/s). A 50-µm-diameter blood vessel phantom is imaged, and $SO_2$ levels are measured using DWP-OCT and compared with values provided by a commercial oximeter at various blood oxygen concentrations. The influences of blood flow speed and mechanisms of SNR phase degradation on the accuracy of $SO_2$ measurement are identified and investigated.

Journal article
Journal of Biomedical Optics