Laser

Custom Ultrafast Lasers

We design and build custom ultrafast lasers to push the limits of traditional multiphoton microscopy and enable advanced multi-wavelength excitation strategies.

Custom Ultrafast Lasers

Diamond Raman laser and Yb fiber amplifier for in vivo multiphoton fluorescence microscopy

Here we introduce a fiber amplifier and a diamond Raman laser that output high powers (6.5 W, 1.3 W) at valuable wavelengths (1060 nm, …

Shaun Engelmann, Annie Zhou, Ahmed Hassan, Michael Williamson, Jeremy Jarrett, Evan Perillo, Alankrit Tomar, David Spence, Theresa Jones, Andrew Dunn

In vivo multiphoton imaging of a diverse array of fluorophores to investigate deep neurovascular structure

We perform high-resolution, non-invasive, in vivo deep-tissue imaging of the mouse neocortex using multiphoton microscopy with a high …

David Miller, Ahmed Hassan, Jeremy Jarrett, Flor Medina, Evan Perillo, Kristen Hagan, S. M. Shams Kazmi, Taylor Clark, Colin Sullender, Theresa Jones, Boris Zemelman, Andrew Dunn

Two-color multiphoton in vivo imaging with a femtosecond diamond Raman laser

Two-color multiphoton microscopy through wavelength mixing of synchronized lasers has been shown to increase the spectral window of …

Evan Perillo, Jeremy Jarrett, Yen-Liang Liu, Ahmed Hassan, Daniel Fernée, John Goldak, Andrei Bonteanu, David J Spence, Hsin-Chih Yeh, Andrew Dunn

Deep in vivo two-photon microscopy with a low cost custom built mode-locked 1060 nm fiber laser

Here we demonstrate that a mode-locked ytterbium fiber laser for two-photon fluorescence microscopy can be built for $13,000. The laser …

Evan Perillo, Justin McCracken, Daniel Fernée, John Goldak, Flor Medina, David Miller, Hsin-Chih Yeh, Andrew Dunn

Improved sensitivity for two-photon frequency-domain lifetime measurement

We demonstrate a method to improve the measurement sensitivity of two-photon frequency-domain lifetime measurements in poor signal to …

Arnold Estrada, Andrew Dunn