Multiphoton Microscopy

We design and build high-resolution microscopes to study the three-dimensional vascular and neuronal structures of the cortex at depths and resolutions pushing the limits of optical imaging.

Explore the intricate structure and function of mouse blood vessels through the lab’s use of Vectorization, Multiphoton Microscopy, and Computational Modeling techniques to create augmented reality models. View our selection of models on your mobile device for an interactive experience.

We developed an automated vectorization method to extract blood vessel geometry and vascular statistics directly from large volumes of unsegmented multiphoton microscopy imagery.

We simulate photon propagation through large cortical tissue geometries in order to study the effects of scattering and develop new imaging techniques based on dynamic light scattering.

We developed imaging systems for use with oxygen-sensitive phosphorescent dyes in order to map cortical oxygen tension (pO2) and study its impact on the progression of ischemic stroke.

We design and build custom ultrafast lasers to push the limits of traditional multiphoton microscopy and enable advanced multi-wavelength excitation strategies.

We built a three-dimensional single particle tracking system capable of spatial localization far beyond the diffraction limit for use in studying molecular transport dynamics.

A potential method for tracking neurovascular disease progression over time in preclinical models is multiphoton fluorescence …

Two-photon microscopy is used routinely for in vivo imaging of neural and vascular structures and functions in rodents with a high …

Here we introduce a fiber amplifier and a diamond Raman laser that output high powers (6.5 W, 1.3 W) at valuable wavelengths (1060 nm, …