Multiphoton Microscopy

Diamond Raman laser and Yb fiber amplifier for in vivo multiphoton fluorescence microscopy
Here we introduce a fiber amplifier and a diamond Raman laser that output high powers (6.5 W, 1.3 W) at valuable wavelengths (1060 nm, …
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Evaluation of resonant scanning as a high-speed imaging technique for two-photon imaging of cortical vasculature
We demonstrate a simple, low-cost two-photon microscope design with both galvo-galvo and resonant-galvo scanning capabilities. We …
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Evaluation of resonant scanning as a high-speed imaging technique for two-photon imaging of cortical vasculature
Segmentation-Less, Automated, Vascular Vectorization
Recent advances in two-photon fluorescence microscopy (2PM) have allowed large scale imaging and analysis of blood vessel networks in …
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Mathematical synthesis of the cortical circulation for the whole mouse brain—part II: Microcirculatory closure
Recent advancements in multiphoton imaging and vascular reconstruction algorithms have increased the amount of data on cerebrovascular …
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Reactive astrocytes facilitate vascular repair and remodeling after stroke
Brain injury causes astrocytes to assume a reactive state that is essential for early tissue protection, but how reactive astrocytes …
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Reactive astrocytes facilitate vascular repair and remodeling after stroke
A Window of Vascular Plasticity Coupled to Behavioral Recovery after Stroke
Stroke causes remodeling of vasculature surrounding the infarct, but whether and how vascular remodeling contributes to recovery are …
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A Window of Vascular Plasticity Coupled to Behavioral Recovery after Stroke
Three-Dimensional Two-Color Dual-Particle Tracking Microscope for Monitoring DNA Conformational Changes and Nanoparticle Landings on Live Cells
Here, we present a three-dimensional two-color dual-particle tracking (3D-2C-DPT) technique that can simultaneously localize two …
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Rehabilitative training interacts with ischemia-instigated spine dynamics to promote a lasting population of new synapses in peri-infarct motor cortex
After subtotal infarcts of primary motor cortex (M1), motor rehabilitative training (RT) promotes improvements in paretic forelimb …
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Improved nondegenerate multiphoton microscopy and axial registration with a reflective objective
Conventional, degenerate multiphoton microscopy (D-MPM) requires the use of a high-numerical-aperture (NA) objective. Nondegenerate MPM …
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Polymer dots enable deep in vivo multiphoton fluorescence imaging of microvasculature
Deep in vivo imaging of vasculature requires small, bright, and photostable fluorophores suitable for multiphoton microscopy (MPM). …
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